Custom Panel Design
Next-Generation Sequencing
Do you need a specialised custom made NGS panel to meet your unique research requirements?
Building your own custom panel can be challenging and probe design needs to be cost effective, as complimentary as possible to avoid off target capture, and encompass genomic structural characteristics such as insertions/deletions, microsatellite instability and fusions.
Whether you are studying human or animal genomics let our team of scientists, with expertise in targeted panel design, help you plan your bespoke project ensuring maximum coverage and sensitivity time after time.
Informed Genomics is a UKAS accredited, ISO15189 approved, company with our laboratories based in Birmingham, UK, focused on providing a premium genomics service, with competitive turnaround times.
Custom panel design – service overview
Panel design
DNA extraction
Quality control
Library preparation
NGS sequencing
Data output
Purpose: human and pet research
Service: custom panel design with NGS
Panel probe size: 50 – 25,000 probes per pool
Additional services
DNA extraction service: (sample submission information)
- Whole blood (fresh)
- Plasma
- Saliva
DNA direct into custom panel library preparation:
- Genomic (gDNA)
- Fixed-formalin paraffin-embedded DNA (FFPE)
- Cell-free DNA (cfDNA)
Technology
Next generation sequencing library preparation:
- Cell3™ Target assay
Sequencing platform:
- Illumina
Data transfer
Secure cloud-based data transfer:
- fastp/FastQC files
- Secure data hosting in DNAnexus for easy retrieval via web portal or CLI
- Hassle free data transfer straight to your AWS (Amazon) S3 buckets
Ordering information
Contact via the Informed Genomics contact form
Quality assurance
UKAS accredited, and ISO15189 approved laboratories
Key benefits
Flexibility
-
Any genome considered; contact us to discuss possibilities
-
Expand your input potential; suitable for all DNA types gDNA, FFPE and cfDNA
-
1 gene, or >1000; scalable panel builds from 50 to 25,000 probes per pool
Robust design and workflow
- Low off-target panel design; ensures uniform coverage across your targeted regions
- Error correction built in; unique molecular identifiers (UMIs) lower false-positive call rates, background noise and increase confidence in your results
- Index hopping risk removed; unique dual indexing (UDI) ensures index assignment per sample is maintained during sequencing
Customer feedback
Connor Woolley from the Tomlinson Group
“Our research group worked with Informed Genomics to carry out targeted sequencing of FFPE samples from a cohort of cancer patients that underwent preoperative chemoradiotherapy.
We wanted to explore how the genetics of these cancers changed during treatment and needed high sensitivity to be able to detect any residual tumour cells in excellent responders.
We chose Informed Genomics due to their experience sequencing FFPE material and ctDNA which also requires very high sensitivity and opted to use a custom targeting panel to cover all of our genes of interest.
The team were incredibly helpful and knowledgeable throughout each step of the process, from panel design to any troubleshooting along the way, and we would certainly recommend them going forward.”
Contact us to find out more about our Custom Panel Design Service
Unit 5
Quinton Business Park
11 Ridgeway
Quinton
Birmingham
B32 1AF