RNA sequencing

Revolutionise your research with our high-precision RNA sequencing services, delivering swift and insightful analysis for a deeper understanding of gene expression and structural dynamics.

Our customisable RNA sequencing services have been designed to support your research with workflow flexibility and assay choice in mind, so whether you want to gain a comprehensive view of the transcriptome, or focus solely on gene expression, we can help.

DNA extraction

RNA extraction

Quality control

Quality control

Library preparation

Library preparation

NGS sequencing

NGS sequencing

Bioinformatics analysis

Bioinformatics analysis

Informed Genomics are a UKAS accredited, ISO15189 approved, company with our laboratories based in Birmingham, UK, focused on providing a premium next generation sequencing service.

RNAseq

service overview

Purpose

  • Transcriptomic research

RNA extraction:

  • Whole blood: PAXgene blood RNA tubes, EDTA blood tubes
  • FFPE tissue: slides, curls, macrodissection from pre-marked slides

Sequencing:

  • Total RNA (human/mouse/rat)
  • 3’ RNA selection
  • RIN values 1.5-10 (as appropriate for the RNA assay)

Sequencing only:

  • Illumina compatible libraries

RNA input amount

  • Flexible – please discuss with us especially if you have low RNA quantities

Flexibility

  • Complete service flexibility – contact us to find the best solution for you

Technology

  • Next generation sequencing library preparation
  • Sequencing platform: Illumina

Indexing options

  • 384 indexing strategy

Bioinformatic output

  • FASTQ and MultiQC report
  • Alignment and count metrics can be provided for human RNAseq

Data transfer

Secure, cloud-based data transfer:

  • Secure data hosting in DNAnexus for easy retrieval via web portal or CLI
  • Hassle free data transfer straight to your AWS (Amazon) S3 buckets

Quality assurance

  • UKAS accredited, and ISO15189  approved laboratories

RNA services

We focus on bulk RNA sequencing, which is the transcriptomic analysis of pooled cell populations, tissue sections or biopsies.

In the process of RNA extraction, cells undergo simultaneous lysis, causing their genetic information to blend together. This blending results in the measurement of average expression data across hundreds or millions of cells, allowing for comparisons between different samples.

See which of our RNAseq services fits your research question and sample type:

Total RNA sequencing

 

Our total RNA sequencing service is a powerful, and comprehensive, approach for analysing the entire RNA content within a biological sample.

Unlike mRNA sequencing, which specifically focuses on messenger RNA, total RNA-seq captures a broader spectrum of RNA molecules, allowing you to explore not only protein-coding genes but also non-coding RNA elements that play crucial roles in gene regulation and cellular processes.

Total RNA-seq is particularly valuable for uncovering complex regulatory networks, understanding global changes in gene expression, and identifying novel RNA species.

N

RIN 1.5-10 accepted

N

Read depth 30-50M per sample

N

Comprehensive overview of all RNA species

N

Contact us to discuss low RNA concentration or alternative sequencing depths

3’ mRNA Selection

 

If your sample is too degraded for traditional mRNA-seq consider sequencing your sample using 3’mRNA-selection.

This approach captures the 50-100 bp region near the mRNA poly(A) tail so, even with RIN values as low as 1.5, gathering gene expression data is not compromised.

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Cost-effective sequencing focusing on gene expression

N

Read depth 5-10M per sample

N

RIN 1.5-10 accepted

N

Targets 50-100 bp from the mRNA poly-A tail

N

Contact us to discuss low RNA concentration input

How can Informed Genomics elevate your research into transcriptomics?

Full flexibility to suit your research requirements

Send us your whole blood/FFPE samples, extracted RNA

Flexible sample input

Discuss with us low RNA concentration options

Choice of RNA assay

Total RNAseq – Total RNA sequencing with ribo-depletion/globin depletion compatible with human/mouse/rat

3’ mRNA-selection – Focus on gene expression (gene counting) with optional Lockbox analysis tool for alignment and differential expression

Secure Cloud based data transfer

Fast turnaround times

Dedicated project support from our team

Customer feedback

3

Kasia from CoSyne Therapeutics:

What specific aspects of our services do you find the most beneficial?

“We mostly used Informed Genomics for sequencing of small projects using NextSeq2000. In the start-up environment what is most important for us is speed of data delivery and that’s what we can always count on while working with your team. With other sequencing providers waiting time is usually 4 or more weeks which makes our projects (especially if they are pilots) difficult to deliver on time but we feel like we can always count on you to get sequencing data quickly and with good quality.

 

Also Louise and Jana has been very helpful, friendly and professional while assisting in choosing right options and troubleshooting issues.

 

Feedback that I also received from our bioinformaticians is that it is absolutely painless and very efficient experience in getting data via DNA Nexus (what they can’t say about other sequencing providers) and they are always happy when I mentioned that particular project will be done with Informed Genomics.”

How would you describe your overall experience with our team?

“Absolutely excellent! We don’t have a single thing that we would change about you or could complain about. Please keep this fantastic service for the future as we really like working with you.”

How has Informed Genomics helped contribute to your work?

“As I mentioned in first question, time is the huge value for our projects so Informed Genomics enormously contributed to our work by providing us sequencing data quickly and in easy to access way. Any minute that our bioinformaticians save on obtaining data from the providers – is already beneficial to whole company. Adding to that, weeks saved by getting data quicker than in other sources – is making us able to achieve goals much quicker.”

Would you recommend Informed Genomics to others? If so, why?

“Yes, absolutely. All the conversions we previously had with your representatives showed that you know your field very well, you are very professional and you are able to help make the best decisions when it comes to Genomics. I will be definitely recommending Informed Genomics to anyone who looks for support in library preparation or sequencing.”

Get in touch

Unit 5
Quinton Business Park
11 Ridgeway
Quinton
Birmingham
B32 1AF