Whole exome sequencing
Whole exome panel for research – focus on what matters
A straightforward and robust whole exome sequencing (WES) workflow:
Whole exome sequencing
Human whole exome content
33 Mb panel (37Mb sequencing footprint)
99% ClinVar variants covered
DNA extraction: (sample submission information)
- Whole blood (fresh)
- FFPE tissue – slides, curls, macrodissection from pre-marked slides
DNA direct into library preparation:
- Genomic (gDNA)
- Fixed-formalin paraffin-embedded DNA (FFPE)
- Cell-free DNA (cfDNA)
- Illumina compatible WES libraries
Contact us to find the best solution for you
- gDNA 10-100 ng
- FFPE 10-200 ng
- cfDNA 10 ng
- NGS library preparation: Cell3™ Target: Whole exome enrichment panel
- Sequencing platform: Illumina
- 384 indexing strategy
- Unique dual indexing (UDIs)
- Unique molecular indexes (UMIs)
Secure, cloud-based data transfer:
- fastp/FastQC files
- Secure data hosting in DNAnexus for easy retrieval via web portal or CLI
- Hassle free data transfer straight to your AWS (Amazon) S3 buckets
Why choose Informed Genomics?
Comprehensive WES data from multiple sample typesAssay compatibility with gDNA, FFPE, or cfDNA3
Low DNA concentrations
Inputs from as little as 10 ng gDNA
Flexible service packages
Take advantage of our DNA extraction option to streamline your project from start to finish
Focused panel design
Superior exome capture providing a cost-efficient WES solution
Optimised sequencing performance
Bait design algorithms ensure streamlined coverage enabling accurate low variant calling
Built-in error suppression
Automatic inclusion of UMIs and UDIs during library preparation remove PCR error and index hopping factors
Kasia from CoSyne Therapeutics:
What specific aspects of our services do you find the most beneficial?
“We mostly used Informed Genomics for sequencing of small projects using NextSeq2000. In the start-up environment what is most important for us is speed of data delivery and that’s what we can always count on while working with your team. With other sequencing providers waiting time is usually 4 or more weeks which makes our projects (especially if they are pilots) difficult to deliver on time but we feel like we can always count on you to get sequencing data quickly and with good quality.
Also Louise and Jana has been very helpful, friendly and professional while assisting in choosing right options and troubleshooting issues.
Feedback that I also received from our bioinformaticians is that it is absolutely painless and very efficient experience in getting data via DNA Nexus (what they can’t say about other sequencing providers) and they are always happy when I mentioned that particular project will be done with Informed Genomics.”
How would you describe your overall experience with our team?
“Absolutely excellent! We don’t have a single thing that we would change about you or could complain about. Please keep this fantastic service for the future as we really like working with you.”
How has Informed Genomics helped contribute to your work?
“As I mentioned in first question, time is the huge value for our projects so Informed Genomics enormously contributed to our work by providing us sequencing data quickly and in easy to access way. Any minute that our bioinformaticians save on obtaining data from the providers – is already beneficial to whole company. Adding to that, weeks saved by getting data quicker than in other sources – is making us able to achieve goals much quicker.”
Would you recommend Informed Genomics to others? If so, why?
“Yes, absolutely. All the conversions we previously had with your representatives showed that you know your field very well, you are very professional and you are able to help make the best decisions when it comes to Genomics. I will be definitely recommending Informed Genomics to anyone who looks for support in library preparation or sequencing.”
1. Rabbani B, Tekin M, Mahdieh N. The promise of whole-exome sequencing in medical genetics. Journal of Human Genetics 2014 59:1. 2013;59(1):5-15. doi:10.1038/jhg.2013.114
2. CCDS database. Accessed November 16, 2023. https://www.ncbi.nlm.nih.gov/projects/CCDS/CcdsBrowse.cgi
3. Cell3TM Target Whole Exome Datasheet v1.2 – Nonacus. Accessed November 16, 2023. https://nonacus.com/download/cell3-target-whole-exome-datasheet-v1-2/